In sterile anthers, abnormal distribution of calcium gran - ules first appeared in callus wall of microspore mother cell (Plate IV-1).
在不育花药中，最早出现的钙颗粒异常分布是在小孢子母细胞的胼胝质壁中积累了较多的钙颗粒。
Place the spleen in a Petri plate with 20 ml of RPMI 10% FBS, cut in two pieces and squeeze each of them between two sterile glass slides to obtain a single cell suspension.
脾脏放置在含有细胞培养用的20毫升10%胎牛血清培养皿里，剪成两瓣，用无菌玻璃的边缘挤压，获得单细胞悬液。
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